A THREE-LEGGED KID WAS BORN IN ETHIOPIA - CASE REPORT
We received a malformed goat kid. The kid was part of a twin gestation, from which two male kids born with one of the twins with morphological abnormalities and underwent regular presented numbers of kid legs. A goat give a twin kid which two of them were male kids with one was three-legged and the second one was normal with four –legged was born in Ethiopia at Gambella University small-scale goat farm at June 2015, which is a rare congenital condition characterized by abnormal development of the body due to a genetic abnormality that occurs in rare cases, only one or more than thousands kids born each year in the world come out with that kind of abnormality/three-legged. The location of the case: In Gambella University small-scale goat farm.
MICROBIOLOGICAL, BIOCHEMICAL AND MOLECULAR IDENTIFICATION (PCR-RFLP-ITS) OF THE YEAST FROM SULTANA GRAPE CULTIVATED IN AIN MERANE (WILAYA OF CHLEF)
Grapes defined as the fruit of the vine, the most cultivated in the world. It is considered a major habitat of many yeast species. This work aims to isolate, purify and characterize species of yeast from the sultana grape (Thompson’s seedless)cultivated in the region of Ain Merane (from Chlef, Algeria). Several tests were performed (microbiological and molecular).Two strains isolated from grape juice obtained by simple grinding.Pichia fermentans and Metschnikowia pulcherrima were confirmed by a genetic approach (ITS-RFLP) using two restriction enzymes (Taq I, and HaeIII), of the region (ITSI, 5.8S rRNA, ITSI) amplified by PCR.
THE IN VITRO EVALUATION OF ANTIBACTERIAL EFFECT OF MARRUBIUM VULGARE L. LEAF EXTRACT GROWN IN ALGERIA
The emergence of antibioresistancebacteria has become a public health problem at the global level. This problem is of growing concern in hospitals. It is therefore necessary to make use of new active substances from medicinal plants. In order to validate their traditional uses, our work aims to evaluate the antibacterial activity of methanolic extract of Marrubium vulgare L. against resistant pathogenic microorganisms.The study was carried outby diffusionmethodandmicro-dilution. Resultsobtained showed that the antibacterial activity varies with the extract concentration and the nature of the germ. The MIC ranged for most strains between 125 and 250 mg/ml.
MOLECULAR IDENTIFICATION WITH PCR_DELTA AND PCR_ITS_RFLP OF YEAST IN THE VINEYARDS OF THE PLAIN GHRISS, GRAPE VARIETIES (MERLOT, CABERNET AND CARIGNAN)
Identification and isolation of yeasts can often be problematic. Our work aims to study the diversity of local native flora yeasts by collecting samples of different varieties of grapes (Merlot, Cabernet and Carignan) which are scattered over the vineyards of the plain of Ghriss (Mascara).The main purpose of this study is to develop new methods of detection of our yeast isolates after a realization of macroscopic and microscopic studies that investigate their morphological and cultural characteristics.A protocol for DNA extraction as well as two methods of molecular detection of fungi has targeted DNAr, a PCR-ITS-RFLP was developed for the region ITS 1-DNAr 5.8 S-ITS 2 and the target PCR-Delta Ty1 retrotransposons (Delta region). They are sensitive enough to detect the biodiversity of different yeast species isolated.The results were revealed considerable phenotypic divergence accompanying the molecular diversity and show that there is a strain variation.Despite this diversity, several cases of dominance and survival of strains were observed and ten genera were identified: Pichia, Saccharomyces, Cryptococcus, Rhodotorula, Leucosporidiella, Brettanomyces, Candida, Clockera, Dekkera and Diplodia.
USE OF PROTEIN ISOLATE FROM FISH WASTE "SARDINA PILCHARDUS" IN THE SYNTHESIS OF A CULTURE MEDIUM
Fish processing is a crucial sector to meet the nutrient needs for humans, but it generates a significant amount of the estimated 50% of total waste. It follows the same time a problem of dumping of waste in different environments, which increases environmental pollution, and in particular the water environment. Our work aims to study the physicochemical quality of sardine waste(edges, heads and guts). Then, we seek to exploit these coproducts as medium culture for microorganisms such as Lactobacillus and E. coli. In this case, two universal mediumswere prepared with the isolate obtained from fish in the place ofpeptones of the conventional mediums.The physicochemical analyzes showed that the isolate obtained has a high biological value (rich in essential amino acids). The results indicate that the use of this isolate must be accompanied with other essential elements of culture (vitamin compounds, essential amino acids, minerals) as growth factors to optimize the growth of bacteria involved. This work allowed us to have a recovery method that reduces the risk of pollution caused by discharges of different fish processing industries and we contribute to the development of biotechnology.